Phenotypic analysis of urothelial exfoliated cells in bladder cancer via microfluidic immunoassays: Sialyl-Tn as a novel biomarker in liquid biopsies

• Author(s): Carvalho, Sandra ; Abreu, Catarina M. ; Ferreira, Dylan ; Lima, Luís ; Santos, Lúcio L. ; Ribeiro, Ricardo ; Grenha, Vânia ; Martínez-Fernández, Mónica ; Duenas, Marta ; Suárez-Cabrera, Cristian ; Paramio, Jesus M. ; Diéguez, Lorena ; Freitas, Paulo P. ; Oliveira, Marta I.
• Date: 2020
• Persistent ID: http://hdl.handle.net/10400.22/16517
• Origin: Repositório Científico do Instituto Politécnico do Porto
• Subject(s): Bladder cancer; Microfluidics; Liquid biopsy; Urine; Sialyl-TnMM
• Document Type: Journal article
• Language: English
• Contributor(s): REPOSITÓRIO P.PORTO

Description

Bladder cancer is the most common malignancy of the urinary tract, having one of the highest recurrence rates and progression from non-muscle to muscle invasive bladder cancer that commonly leads to metastasis. Cystoscopy and urine cytology are the standard procedures for its detection but have limited clinical sensitivity and specificity. Herein, a microfluidic device, the UriChip, was developed for the enrichment of urothelial exfoliated cells from fresh and frozen urine, based on deformability and size, and the cancer-associated glycan Sialyl-Tn explored as a putative bladder cancer urinary biomarker.