Author(s):
Sonon, Paulin ; Sadissou, Ibrahim ; Tokplonou, Léonidas ; M'po, Kuumaaté K.G. ; Glitho, Sonya S.C. ; Agniwo, Privat ; Ibikounlé, Moudachirou ; Massaro, Juliana Doblas ; Massougbodji, Achille ; Moreau, Philippe ; Sabbagh, Audrey ; Mendes-Junior, Celso T. ; Moutairou, Kabirou A. ; Castelli, Erick C. [UNESP] ; Courtin, David ; Donadi, Eduardo A.
Date: 2019
Persistent ID: http://hdl.handle.net/11449/189672
Origin: Oasisbr
Subject(s): African; Haplotypes; HLA-E; HLA-F; HLA-G; Massive parallel sequencing
Description
Made available in DSpace on 2019-10-06T16:48:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01
HLA-G/E/F genes exhibit immunomodulatory properties and are expressed in placenta. Little attention has been devoted to the study of these genes in sub-Saharan African populations, which are yet the most diverse. To fill this gap, we evaluated the complete gene variability, approximately 5.1 kb for HLA-G (n = 149), 7.7 kb for HLA-E (n = 150) and 6.2 kb for HLA-F (n = 152) in the remote Beninese Toffin population, using massive parallel sequencing. Overall, 96, 37 and 68 variable sites were detected along the entire HLA-G, -E and -F, respectively, arranged into region-specific haplotypes; i.e., promoter haplotypes (16, 19, and 15 respectively), coding haplotypes (19, 15, and 29 respectively), 3’ untranslated region (3′UTR) haplotypes (12, 7 and 2, respectively) and extended haplotypes (33, 31 and 32 respectively). All promoter/coding/3'UTR haplotypes followed the patterns already described in worldwide populations. HLA-E was the most conserved, exhibiting mainly two full-length encoded-molecules (E*01:01 and E*01:03), followed by HLA-F, three full-length proteins (F*01:01, F*01:02 and F*01:03) and HLA-G, four proteins: three full-length (G*01:01, G*01:03 and G*01:04) and one truncated (G*01:05N). Although HLA-G/E/F alleles in the Toffin population were the most frequently observed worldwide, the frequencies of the coding haplotypes were closely similar to those described for other African populations (Guinea-Conakry and Burkina-Faso), when compared to non-African ones (Brazilian), indicating that variable sites along these genes were present in Africa before human dispersion.
Laboratório de Biologia Molecular Universidade de São Paulo Programa de Imunologia Básica e Aplicada (IBA) Faculdade de Medicina de Ribeirão Preto (FMRP-USP)
Laboratório de Biologia Molecular Universidade de São Paulo Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto (FMRP-USP)
Centre d'Etude et de Recherche sur le Paludisme Associé à la Grossesse et à l'Enfance (CERPAGE) Faculté des Sciences de la Santé
UMR 216 MERIT IRD Faculté de Pharmacie de Paris Université Paris Descartes Sorbonne Paris Cité
Université d'Abomey-Calavi
Département de Zoologie Faculté des Sciences et Techniques Université d'Abomey-Calavi
Commissariat à l'Energie Atomique et aux Energies Alternatives Direction de la Recherche Fondamentale Institut de Biologie François Jacob Service de Recherches en Hémato-Immunologie Hôpital Saint-Louis
Université Paris-Diderot Sorbonne Paris-Cité UMR_E5 Institut Universitaire d'Hématologie Hôpital Saint-Louis
Departamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo
Laboratoire de Biologie et Physiologie Cellulaire Université d'Abomey-Calavi
São Paulo State University (UNESP) Molecular Genetics and Bioinformatics Laboratory Experimental Research Unit (UNIPEX) School of Medicine
São Paulo State University (UNESP) Department of Pathology School of Medicine
São Paulo State University (UNESP) Molecular Genetics and Bioinformatics Laboratory Experimental Research Unit (UNIPEX) School of Medicine
São Paulo State University (UNESP) Department of Pathology School of Medicine
