Author(s):
Oliveira, Ana Isabel ; Rodrigues, Luísa Cidália Guimarães ; Soares da Costa, Diana ; Fernandes, E. M. ; Reis, R. L. ; Neves, N. M. ; Leão, P. ; Martins, Albino
Date: 2024
Persistent ID: https://hdl.handle.net/1822/88522
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/OE/PD%2FBDE%2F142979%2F2018/PT;
info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F93697%2F2013/PT;
info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F85790%2F2012/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50026%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50026%2F2020/PT;
Subject(s): Colorectal; electrospun fibrous meshes; Etoricoxib; Inflammation
Description
Selective COX-2 inhibitors such as etoricoxib (ETX) are potentially indicated for the treatment of intestinal inflammatory disorders. However, their systemic administration provokes some off-site secondary effects, decreasing the desirable local effectiveness. To circumvent such limitations, herein an ETX delivery system based on electrospun fibrous meshes (eFMs) was proposed. ETX at different concentrations (1, 2, and 3 mg mL−1) was loaded into eFMs, which not affect the morphology and the mechanical properties of this drug delivery system (DDS). The ETX showed a burst release within the first 12 h, followed by a faster release until 36 h, gradually decreasing over time. Importantly, the ETX studied concentrations were not toxic to human colonic cells (i.e. epithelial and fibroblast). Moreover, the DDS loading the highest concentration of ETX, when tested with stimulated human macrophages, promoted a reduction of PGE2, IL-8 and TNF-α secretion. Therefore, the proposed DDS may constitute a safe and efficient treatment of colorectal diseases promoted by inflammatory disorders associated with COX-2.
The authors thank the Portuguese Foundation for Science and Technology (FCT) and CUF, S.A. for the Ph.D. scholarships of A. Oliveira (PD/BDE/142979/2018 and COVID/BDE/152779/2022). The authors also thank the FCT for the financial support of L. C. Rodrigues (SFRH/BPD/93697/2013), D. Soares da Costa (SFRH/BPD/85790/2012) and the projects UIDB/50026/2020, and UIDP/50026/2020. This work was also financially supported by the project Bluebiolab – Transboundary Marine Biotechnology Laboratory (0474_BLUEBIOLAB_1_E) financed by the Interreg programme Spain-Portugal through the European Regional Development Fund (ERDF). Furthermore, the authors would like to thank the contributions to this research from the project TERM RES Hub – Scientific Infrastructure for Tissue Engineering and Regenerative Medicine, reference PINFRA/22190/2016 (Norte-01-0145-FEDER022190), funded by FCT in cooperation with the Northern Portugal Regional Coordination and Development Commission (CCDR-N), for providing relevant lab facilities, state-of-the art equipment and highly qualified human resources.
