Author(s):
Mendes, Filipa Diana Silva ; Santos-Pereira, Cátia ; Vieira, Tatiana F. ; Pinto, Mélanie Martins ; Castro, Bruno B. ; Sousa, Sérgio F. ; Sousa, Maria João ; Devin, Anne ; Chaves, Susana Alexandra Rodrigues
Date: 2024
Persistent ID: https://hdl.handle.net/1822/92450
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04050%2F2020/PT;
Subject(s): Cymoxanil; Cytochrome c; Cytochrome c oxidase; Mode of action; Respiration
Description
Cymoxanil (CYM) is a widely used synthetic acetamide fungicide, but its biochemical mode of action remains elusive. Since CYM inhibits cell growth, biomass production, and respiration in Saccharomyces cerevisiae, we used this model to characterize the effect of CYM on mitochondria. We found it inhibits oxygen consumption in both whole cells and isolated mitochondria, specifically inhibiting cytochrome c oxidase (CcO) activity during oxidative phosphorylation. Based on molecular docking, we propose that CYM blocks the interaction of cytochrome c with CcO, hampering electron transfer and inhibiting CcO catalytic activity. Although other targets cannot be excluded, our data offer valuable insights into the mode of action of CYM that will be instrumental in driving informed management of the use of this fungicide.
FCT -Fundação para a Ciência e a Tecnologia(UIDB/04050/2020)
info:eu-repo/semantics/publishedVersion
