Autor(es): Almeida, Raquel Carvalho Ferreira Costa ; Franco, Albina Ribeiro ; Pesqueira, Tamagno ; Oliveira, Mariana Braga ; Babo, Pedro S. ; Leonor, I. B. ; Mano, J. F. ; Reis, R. L. ; Gomes, Manuela E.
Data: 2018
Identificador Persistente: https://hdl.handle.net/1822/51342
Origem: RepositóriUM - Universidade do Minho
Assunto(s): Antibacterial efficacy; Controlled release; Endogenous regeneration; Tenogenic genes; Tendon patch
Platelet-derived biomaterials are widely explored as cost-effective sources of therapeutic factors, holding a strong potential for endogenous regenerative medicine. Particularly for tendon repair, treatment approaches that shift the injury environment are explored to accelerate tendon regeneration. Herein, genipin-crosslinked platelet lysate (PL) patches are proposed for the delivery of human-derived therapeutic factors in patch augmentation strategies aiming at tendon repair. Developed PL patches exhibited a controlled release profile of PL proteins, including bFGF and PDGF-BB. Additionally, PL patches exhibited an antibacterial effect by preventing the adhesion, proliferation and biofilm formation by S. aureus, a common pathogen in orthopaedic surgical site infections. Furthermore, these patches supported the activity of human tendon-derived cells (hTDCs). Cells were able to proliferate over time and an up-regulation of tenogenic genes (SCX, COL1A1 and TNC) was observed, suggesting that PL patches may modify the behavior of hTDCs. Accordingly, hTDCs deposited tendon-related extracellular matrix proteins, namely collagen type I and tenascin C. In summary, PL patches can act as a reservoir of biomolecules derived from PL and support the activity of native tendon cells, being proposed as bioinstructive patches for tendon regeneration.
