Autor(es): George, S. ; Dong, C. ; Aaron, K. ; Sherman, A. ; Van Gerwen, O. ; Tamhane, A. ; Taylor, C. ; Sousa, Lúcia Filipa Guimarães Vieira ; Almeida, C. ; Cerca, Nuno ; Muzny, C.
Data: 2024
Identificador Persistente: https://hdl.handle.net/1822/94181
Origem: RepositóriUM - Universidade do Minho
Objective Key BV-associated bacteria that may be important in incident BV (iBV) pathogenesis are Gardnerella species and Prevotella bivia. We quantified the amount of these bacterial species in clue cell biofilms in longitudinal vaginal specimens from iBV cases and controls using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH). Study Design Women with optimal vaginal microbiota self-collected twice daily vaginal specimens for 60 days. iBV was defined as a Nugent score of 7-10 on 4 consecutive specimens. For women who developed iBV (cases), specimens for 14 days pre-iBV, day of iBV, and 3 days post-iBV were selected. Controls were selected based on age, race, contraceptive method, and menstrual cycle day. Fluorescent PNA probes were used to quantify Gardnerella spp. and P. bivia in specimens. Six epi-fluorescent microscopic images Results Of 110 women enrolled, 10 developed iBV (9.1%); PNA-FISH is completed for 5 cases and 5 controls (Table 1). Pooled Gardnerella spp. counts in cases spiked on days -5.5 to -4 prior to iBV, decreased on the day of iBV, and spiked again on days 1-3 post iBV. Pooled P. bivia counts in cases followed a similar pattern to Gardnerella spp., but this pattern was more subtle due to smaller ranges of the count data. Conclusion P. bivia was detected in clue cell biofilms, though in smaller amounts than Gardnerella spp. Spikes in counts may suggest when key events in iBV pathogenesis may be occurring.
