Author(s):
Mateus, Rita ; Lourenço, Raquel ; Fang, Y. ; Brito, G ; Farinho, Ana ; Valerio, Fabio ; Jacinto, Antonio
Date: 2015
Persistent ID: http://hdl.handle.net/10362/26612
Origin: Repositório Institucional da UNL
Project/scholarship:
info:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F62126%2F2009/PT;
info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBEX-BID%2F1176%2F2012/PT;
Subject(s): EXPRESSION; CONTACT INHIBITION; PROTEIN YAP; TRANSCRIPTIONAL COACTIVATOR; Zebrafish; Cell density; Hippo/Yap; PROLIFERATION; APOPTOSIS; CATENIN; DROSOPHILA; Fin regeneration; F-actin; INDUCTION; HIPPO SIGNALING PATHWAY
Description
This work was supported by funding from Fundacao para a Ciencia e Tecnologia [SFRH/BD/62126/2009, PTDC/BEX-BID/1176/2012]; and Agence Nationale de la Recherche [ANR-11-BSV5-0021].
Caudal fin regeneration is characterized by a proliferation boost in the mesenchymal blastema that is controlled precisely in time and space. This allows a gradual and robust restoration of original fin size. However, how this is established and regulated is not well understood. Here, we report that Yap, the Hippo pathway effector, is a chief player in this process: functionally manipulating Yap during regeneration dramatically affects cell proliferation and expression of key signaling pathways, impacting regenerative growth. The intracellular location of Yap is tightly associated with different cell densities along the blastema proximal-distal axis, which correlate with alterations in cell morphology, cytoskeleton and cell-cell contacts in a gradient-like manner. Importantly, Yap inactivation occurs in high cell density areas, conditional to F-actin distribution and polymerization. We propose that Yap is essential for fin regeneration and that its function is dependent on mechanical tension, conferred by a balancing act of cell density and cytoskeleton activity.
