Author(s): Leal, Tânia ; Abrunhosa, Luís ; Domingues, Lucília ; Venâncio, Armando ; Oliveira, Carla
Date: 2019
Persistent ID: http://hdl.handle.net/1822/60971
Origin: RepositóriUM - Universidade do Minho
Project/scholarship: info:eu-repo/grantAgreement/FCT/5876/147337/PT;
Subject(s): Ochratoxin A; bovine serum albumin; solid-phase extraction; wine analysis; method validation; Science & Technology
Analytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2=0.9999) in the OTA concentration range studied (0.05 to 3.0 g L-1), with recovery rates above 98%. It also showed low detection (0.017 g L-1) and quantification (0.051 g L-1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results.
This study was supported by the Portuguese Foundation for Scienceand Technology (FCT) under the scope of the strategic funding of UID/BIO/04469 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684)and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Luís Abrunhosawas supported by grant UMINHO/BPD/51/2015 from project UID/BIO/04469/2013 financed by FCT/MEC (OE). Carla Oliveira was sup-ported by the FCT grant SFRH/BPD/110640/2015.
info:eu-repo/semantics/publishedVersion
