Autor(es): Fernandez-Bertolez, N. ; Touzani, A. ; Martinez, L. ; Méndez, J. ; Ramos-Paz, L. ; Reis, A.T. ; Fraga, S. ; Costa, C. ; Teixeira, J.P. ; Pásaro, E. ; Valdiglesias, V. ; Laffon, B.
Data: 2023
Identificador Persistente: http://hdl.handle.net/10400.18/9076
Origem: Repositório Científico do Instituto Nacional de Saúde
Assunto(s): Cerium Dioxide Nanoparticles; MTT Assay; γH2AX Assay; Glial Cells; Neuronal Cells; Genotoxicidade Ambiental
Cerium dioxide nanoparticles (CeO2 NP) show antioxidant enzyme mimetic properties and free radical scavenging activity. These properties make them a promising material for biomedical applications, but their potential adverse effects are not totally understood yet. Our objective was to assess the biological behaviour of CeO2 NP in human neuronal and glial cells. After carrying out the physical-chemical characterization of the CeO2 NP and analysing their ability to be taken up by neuronal and glial cells, the possible alterations in cell viability and induction of DNA double strand breaks were determined by means of MTT assay and γH2AX assay, respectively. The possible existence of interference of the NP with the assay methodologies was previously addressed and corrected when necessary. The results obtained showed that, even though there was a significant dose- and time-dependent internalization of the NP by both cell lines, the CeO2 NP generally presented scarce cyto- or genotoxicity, essentially depending on the NP exposure time and being restricted to higher doses. These results provide a better understanding of the interaction of CeO2 NP with cellular systems and their possible adverse effects, specifically at nervous system level.
