Author(s): Carrapita, J ; Abrantes, AM ; Campelos, S ; Gonçalves, AC ; Cardoso, D ; Sarmento-Ribeiro, AB ; Rocha, C ; Santos, JN ; Botelho, MF ; Tralhão, JG ; Farges, O ; Barbosa, JM
Date: 2016
Persistent ID: http://hdl.handle.net/10400.4/1961
Origin: Repositório do Centro Hospitalar e Universitário de Coimbra
Subject(s): Artéria Esplénica; Hepatectomia
It was reported that prevention of acute portal overpressure in small-for-size livers by inflow modulation results in a better postoperative outcome. The aim is to investigate the impact of portal blood flow reduction by splenic artery ligation after major hepatectomy in a murine model. Forty-eight rats were subjected to an 85% hepatectomy or 85% hepatectomy and splenic artery ligation. Both groups were evaluated at 24, 48, 72 and 120 post-operative hours: liver function, regeneration and viability. All methods and experiments were carried out in accordance with Coimbra University guidelines. Splenic artery ligation produces viability increase after 24 h, induces a relative decrease in oxidative stress during the first 48 hours, allows antioxidant capacity increment after 24 h, which is reflected in a decrease of half-time normalized liver curve at 48 h and at 72 h and in an increase of mitotic index between 48 h and 72 h. Splenic artery ligation combined with 85% hepatectomy in a murine model, allows portal inflow modulation, promoting an increase in hepatocellular viability and regeneration, without impairing the function, probably by inducing a less marked elevation of oxidative stress at first 48 hours.
