Author(s):
Brilhante, Raimunda Sâmia Nogueira ; Silva, Natalya Fechine ; Lima, Rita Amanda Chaves de ; Caetano, Érica Pacheco ; Alencar, Lucas Pereira de ; Castelo-Branco, Débora de Souza Collares Maia ; Moreira, José Luciano Bezerra ; Bandeira, Silviane Praciano ; Camargo, Zoilo Pires de ; Rodrigues, Anderson Messias ; Bandeira, Tereza de Jesus Pinheiro Gomes ; Monteiro, André Jalles ; Cordeiro, Rossana de Aguiar ; Sidrim, José Júlio Costa ; Rocha, Marcos Fábio Gadelha
Date: 2016
Origin: Oasisbr
Subject(s): Sporothrix; Glicerol; Lactose
Description
The present study evaluated the maintenance of Sporothrix spp. (6 Sporothrix brasiliensis;6S. schenckii;5 S. mexicana, and 3 S. globosa) in saline at 4 C, and in 10% glycerol plus either 10% lactose or 10% sucrose, at -20 C and -80 C. Viability was assessed after 3, 6, and 9 months of storage, through the recovery of strains on potato dextrose agar and analysis of macro- and micromorphological features. Conidium quantification was performed before and after storage, at 3, 6 and 9 months. 100% viability was observed, regardless of storage conditions or time period. Storage at 4 C and at -20 C did not alter the number of conidia, but lower conidium counts were observed at -80 C. This study shows that the combination of glycerol with lactose or sucrose is effective to maintain Sporothrix spp. at freezing temperatures.
