Autor(es):
Plácido, Alexandra ; Bueno, João ; Barbosa, Eder A. ; Moreira, Daniel C. ; Dias, Jhones Do Nascimento ; Cabral, Wanessa Felix ; Albuquerque, Patrícia ; Bessa, Lucinda J. ; Freitas, Jaime ; Kuckelhaus, Selma A. S. ; Lima, Filipe C. D. A. ; Batagin-Neto, Augusto [UNESP] ; Brand, Guilherme D. ; Relvas, João B. ; Leite, José Roberto S. A. ; Eaton, Peter
Data: 2020
Identificador Persistente: http://hdl.handle.net/11449/200233
Origem: Oasisbr
Assunto(s): Antioxidant peptides; Bioactive molecules; Portuguese biodiversity; Salamandra salamandra; Antioxidant peptides; Antioxidant peptides; Bioactive molecules; Bioactive molecules; Portuguese biodiversity; Portuguese biodiversity; Salamandra salamandra; Salamandra salamandra
Descrição
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Universidade Estadual Paulista
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação para a Ciência e a Tecnologia
Amphibian skin is a multifunctional organ that plays key roles in defense, breathing, and water balance. In this study, skin secretion samples of the fire salamander (Salamandra salamandra) were separated using RP-HPLC and de novo sequenced using MALDI-TOF MS/MS. Next, we used an in silico platform to screen antioxidant molecules in the framework of density functional theory. One of the identified peptides, salamandrin-I, [M + H]+ = 1406.6 Da, was selected for solid-phase synthesis; it showed free radical scavenging activity against DPPH and ABTS radicals. Salamandrin-I did not show antimicrobial activity against Gram-positive and-negative bacteria. In vitro assays using human microglia and red blood cells showed that salamandrin-I has no cytotoxicity up to the concentration of 100 µM. In addition, in vivo toxicity tests on Galleria mellonella larvae resulted in no mortality at 20 and 40 mg/kg. Antioxidant peptides derived from natural sources are increasingly attracting interest. Among several applications, these peptides, such as salamandrin-I, can be used as templates in the design of novel antioxidant molecules that may contribute to devising strategies for more effective control of neurological disease.
LAQV/REQUIMTE Department of Chemistry and Biochemistry Faculty of Sciences University of Porto
Institute for Research and Innovation in Health (i3S) and Institute for Molecular and Cell Biology IBMC
Center for Research in Applied Morphology and Immunology NuPMIA University of Brasilia, DF
Laboratory for the Synthesis and Analysis of Biomolecules (LSAB) Institute of Chemistry University of Brasília, DF
Laboratory of Molecular Biology of Fungal Pathogens Department of Cell Biology Institute of Biological Sciences University of Brasília, DF
Institute for Research and Innovation in Health (i3S) and National Institute of Biomedical Engineering INEB
Federal Institute of Education Science and Technology of São Paulo, Campus Matão
São Paulo State University (UNESP), Campus of Itapeva
São Paulo State University (UNESP), Campus of Itapeva
CAPES: 001
CNPq: 420449/2018-3
CNPq: 428211/2018-6
Fundação para a Ciência e a Tecnologia: PTDC/BII_BIO/31158/2017
