Autor(es):
Resende, Lucilene Aparecida ; Roatt, Bruno Mendes ; Soares, Rodrigo Dian de Oliveira Aguiar ; Viana, Kelvinson Fernandes ; Mendonça, Ludmila Zanandreis de ; Lanna, Mariana Ferreira ; Lemos, Denise da Silveira ; Oliveira, Rodrigo Corrêa de ; Martins Filho, Olindo Assis ; Fujiwara, Ricardo Toshio ; Carneiro, Cláudia Martins ; Reis, Alexandre Barbosa ; Giunchetti, Rodolfo Cordeiro
Data: 2015
Origem: Oasisbr
Assunto(s): Vaccine; Canine visceral leishmaniasis; Cytokines; immunoprotection; Leishmania chagasi
Descrição
In the studies presented here, dogs were vaccinated against Leishmania (Leishmania) cha-gasi challenge infection using a preparation of Leishmania braziliensis promastigote proteinsand saponin as adjuvant (LBSap). Vaccination with LBSap induced a prominent type 1immune response that was characterized by increased levels of interleukin (IL-) 12 andinterferon gamma (IFN- _) production by peripheral blood mononuclear cells (PBMC) uponstimulation with soluble vaccine antigen. Importantly, results showed that this type ofresponsiveness was sustained after challenge infection; at day 90 and 885 after L. chagasichallenge infection, PBMCs from LBSap vaccinated dogs produced more IL-12, IFN- _ andconcomitant nitric oxide (NO) when stimulated with Leishmania antigens as comparedto PBMCs from respective control groups (saponin, LB- treated, or non-treated controldogs). Moreover, transforming growth factor (TGF)- _ decreased in the supernatant of SLcA-stimulated PBMCs in the LBSap group at 90 days. Bone marrow parasitological analysisrevealed decreased frequency of parasitism in the presence of vaccine antigen. It is con-cluded that vaccination of dogs with LBSap vaccine induced a long-lasting type 1 immuneresponse against L. chagasi challenge infection.
