Author(s): Souto, Eliana B. ; Zielinska, Aleksandra ; Souto, Selma B. ; Durazzo, Alessandra ; Lucarini, Massimo ; Santini, Antonello ; Silva, Amélia M. ; Atanasov, Atanas G. ; Marques, Conrado ; Andrade, Luciana N. ; Severino, Patricia
Date: 2020
Persistent ID: https://hdl.handle.net/1822/64150
Origin: RepositóriUM - Universidade do Minho
Subject(s): (+)-limonene 1; 2-epoxide; monoterpene; Imwitor 900K-SLN; lipid peroxidation; cytotoxicity; HaCaT cell line; (+)-limonene 1,2-epoxide
In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed.
