Author(s): Cavalcante, Jânio Emanuel Andrade ; Machado, Rosângela Pinheiro Gonçalves ; Laurentino, Marília Rocha ; Santos, Talyta Ellen de Jesus dos ; Bandeira, Izabel Cristina Justino ; Maia Filho, Pedro Aurio ; Figueiredo, Monalisa Feliciano ; Martins, Alice Maria Costa ; Lemes, Romélia Pinheiro Gonçalves
Date: 2016
Origin: Oasisbr
Subject(s): Interleucina-10; Anemia
Objective/background: Sickle-cell anemia (SCA) is a genetic blood disease characterized by chronic inflammation and a heterogeneous clinical picture. Serum tumor necrosis factor (TNF-alpha) and interleukin 10 (IL-10) levels are associated with the clinical course of SCA. This study aimed to evaluate the association between the frequency of the polymorphisms TNF- alpha-308 G ? A, IL-10-1082 G ? A, IL-10-819 C ? T, and IL-10-592 A ? C; serum TNF-alpha; and IL-10 levels, and the incidence of clinical events in SCA patients. Methods: Polymerase chain reaction–restriction fragment length polymorphism and enzyme- linked immunosorbent assay were performed on 25 adults with SCA at the steady state; their data were compared with those for 26 healthy individuals. Results: The most frequent genotype of the TNF-alpha polymorphism was GG (low producer), and the most frequent genotype of the IL-10 polymorphisms was ‘‘low producer ” (ACC ACC, ACC ATA, ATA ATA). The TNF-alpha levels were significantly higher in SCA in patients with acute chest syndrome (ACS). The IL-10 levels were reduced in polytransfusion and in patients with ACS. Conclusion: The patients presented prevalence of TNF-alpha and IL-10 low-profile producer. The cytokine serum levels presented an association with the presence of polytransfusion and ACS in SCA patients.
