Author(s): Pinto, Natália Bitú ; Morais, Talita Cavalcante ; Carvalho, Karine Maria Martins Bezerra ; Silva, Cecília Rocha ; Andrade, Geanne Matos de ; Brito, Gerly Anne de Castro ; Veras, Maria Leopoldina ; Pessoa, Otília Deusdênia Loiola ; Rao, Vietla Satyanarayana ; Santos, Flávia Almeida
Date: 2013
Origin: Oasisbr
Subject(s): Antígeno Polipeptídico Tecidual; Dermatite; Ratos
The anti-inflammatory effect of physalin E, a seco-steroid isolated from Physalis angulata L. was evaluated on acute and chronic models of dermatitis induced by 12-O-tetradecanoyl-phorbol-13- acetate (TPA) and oxazolone, respectively, in mouse ear. The changes in ear edema/thickness, production of pro-inflammatory cytokines (TNF- a and IFN- g ), myeloperoxidase (MPO) activity, and histological and immunohistochemical findings were analysed, as indicators of dermal inflammation. Similar to dexamethasone, topically applied Physalin E (0.125; 0.25 and 0.5 mg/ear) potently inhibited the TPA and oxazolone-induced dermatitis, leading to substantial reductions in ear edema/thickness, pro-inflammatory cytokines, and MPO activity. These effects were reversed by mifepristone, a steroid antagonist and confirmed by immunohistochemical and histopathological analysis. The data suggest that physalin E may be a potent and topically effective anti-inflammatory agent useful to treat the acute and chronic skin inflammatory conditions.
