Author(s): Silva-Vaz, Pedro ; Jarak, Ivana ; Rato, Luis ; Oliveira, Pedro F. ; Morgado-Nunes, Sara ; Paulino, Aida ; Castelo-Branco, Miguel ; Botelho, Filomena ; Tralhão, José Guilherme ; Alves, Marco G. ; Abrantes, Ana Margarida
Date: 2021
Persistent ID: https://hdl.handle.net/10316/103684
Origin: Estudo Geral - Universidade de Coimbra
Subject(s): acute biliary pancreatitis; inflammation; prognostic; severity; hepcidin; systemic inflammatory response index; oxidative stress; metabonomics
Acute pancreatitis (AP) is an inflammatory process of the pancreas with variable involvement of the pancreatic and peripancreatic tissues and remote organ systems. The main goal of this study was to evaluate the inflammatory biomarkers, oxidative stress (OS), and plasma metabolome of patients with different degrees of biliary AP severity to improve its prognosis. Twenty-nine patients with biliary AP and 11 healthy controls were enrolled in this study. We analyzed several inflammatory biomarkers, multifactorial scores, reactive oxygen species (ROS), antioxidants defenses, and the plasma metabolome of biliary AP and healthy controls. Hepcidin (1.00), CRP (0.94), and SIRI (0.87) were the most accurate serological biomarkers of AP severity. OS played a pivotal role in the initial phase of AP, with significant changes in ROS and antioxidant defenses relating to AP severity. Phenylalanine (p < 0.05), threonine (p < 0.05), and lipids (p < 0.01) showed significant changes in AP severity. The role of hepcidin and SIRI were confirmed as new prognostic biomarkers of biliary AP. OS appears to have a role in the onset and progression of the AP process. Overall, this study identified several metabolites that may predict the onset and progression of biliary AP severity, constituting the first metabonomic study in the field of biliary AP.
This work was supported by the Foundation for Science and Technology (FCT), Portugal through financial support (Strategic Projects UID/NEU/04539/2019, UIDB/04539/2020 and UIDP/04539/2020 (CIBB) and FCT: M.G.Alves (IFCT2015), UMIB (UIDB/00215/2020 and UIDP/00215/2020); NMR data was collected at the UC-NMR facility which is supported in part by FEDER-European Regional Development Fund through the COMPETE Programme (Operational Programme for Competitiveness) and by National Funds through FCT through grants REEQ/481/QUI/2006, RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012, and Rede Nacional de Ressonância Magnética Nuclear (RNRMN).
