Autor(es): Araújo, Márcio Sobreira Silva ; Andrade, Renata Aline de ; Avelar, Renato Sathler ; Carvalho, Andréa Teixeira de ; Andrade, Mariléia Chaves ; Vianna, Leonardo Rocha ; Mayrink, Wilson ; Reis, Alexandre Barbosa ; Malaquias, Luiz Cosme Cotta ; Melo, Maria Norma ; Martins Filho, Olindo Assis
Data: 2012
Origem: Oasisbr
Assunto(s): Canine visceral leishmaniasis; Leishvaccine; Leishmune; Cytokines; Nitric oxide
It is generally accepted that distinct cytokine expression by the cellular immune response plays a critica role during the outcome of experimental as well as natural canine visceral Leishmaniasis (CVL). Despite the fact that immunoprophylaxis of CVL has become an important control strategy and protective immunity has been reported upon immunization with whole as well as purifiedLeishmaniaantigens, the cytokine profile of T-cells triggered by anti-CVL vaccines still remain to be determined. Herein, we have developed a cross-sectional analysis of German Shepherd dogs submitted to vaccination protocols with Leishvaccine (n = 6) and Leishmune ® (n = 6). Our data identified distinct immunological profiles elicited by Leishvaccine and Leishmune ® , with the Leishvaccine triggering a mixed, IFN- and IL-4, cytokine pattern in addition to high levels of anti- LeishmaniaIgG1, whereas the Leishmune ® induced an immunological pattern char- acterized by enhanced levels of IFN- , NO and anti- Leishmania chagasi IgG2. It was important to notice that despite the distinct immunological patterns triggered by Leishvaccine and Leishmune ® , the ability of both immunobiologicals to activate T-cell-derived IFN- synthesis further suggesting their immunogenic potential against CVL. These findings added support to our hypothesis that both antigenic composition (whole antigen in Leishvaccine versus purified antigen in Leishmune ® ) as well as the adjuvant nature (BGC and saponin) used for the vaccine formulation may count for the distinct activation pattern observed.
