Author(s):
Miranda,Silvana Spindola de ; Almeida,Isabela Neves de ; Mansur,Maria de Fátima Filardi Oliveira ; Figueredo,Lida Jouca de Assis ; Carvalho,Wânia da Silva ; Hadaad,João Paulo Amaral ; Diniz,Jaciara de Lourdes do Carmo Guimarães ; Groll,Andrea von ; Silva,Pedro Almeida da ; Lopes,Maria Luiza ; Santos,Marcelo Cordeiro dos ; Brito,Alexandra ; Mello,Fernanda Carvalho de Queiroz ; Malaquias,Thiago da Silva Santos ; Croda,Julio ; Pinhata,Juliana Maira Watanabe ; Oliveira,Rosângela Siqueira de ; Chimara,Erica ; Rossetti,Maria Lúcia ; Halon,Maria Laura ; Lourenço,Maria Cristina ; Medeiros,Reginalda Ferreira de Melo ; Montes,Fátima Cristina Onofre Fandinho ; Machado,Diana ; Viveiros,Miguel ; Kritski,Afrânio Lineu
Date: 2020
Origin: Oasisbr
Subject(s): tuberculosis; MDR; nitrate reductase; technology
Description
Abstract (1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.
