Autor(es):
Sande, Maria Georgina ; Roque, Lúcia ; Braga, Adelaide Correia ; Marques, Márcia ; Ferreira, Débora Carina Gonçalves Abreu ; Saragliadis, Athanasios ; Rodrigues, Joana Lúcia Lima Correia ; Linke, Dirk ; Ramada, David ; Silva, C. ; Rodrigues, L. R.
Data: 2023
Identificador Persistente: https://hdl.handle.net/1822/80471
Origem: RepositóriUM - Universidade do Minho
Assunto(s): Adhesins; Arginine magnetic nanoparticles; Bacterial adhesion; Enrichment; Hydrolyzed collagen magnetic nanoparticles; Ubiquitous surface protein A2 (UspA2); Yersinia adhesin A (YadA)
Descrição
Enrichment and diagnosis tools for pathogens currently available are time consuming, thus the development of fast and highly sensitive alternatives is desirable. In this study, a novel approach was described that enables selective capture of bacteria expressing hydrolyzed collagen-binding adhesins with hydrolyzed collagen-coated magnetic nanoparticles (MNPs). This platform could be useful to shorten the time needed to confirm the presence of a bacterial infection. MNPs were synthesized by a simple two-step approach through a green co-precipitation method using water as solvent. These MNPs were specifically designed to interact with pathogenic bacteria by establishing a hydrolyzed collagen-adhesin linker. The bacterial capture efficacy of hydrolyzed collagen MNPs (H-Coll
