Author(s): Vital, Wendel Coura ; Carneiro, Cláudia Martins ; Martins, Helen Rodrigues ; Lana, Marta de ; Veloso, Vanja Maria ; Carvalho, Andréa Teixeira de ; Bahia, Maria Terezinha ; Oliveira, Rodrigo Corrêa de ; Martins Filho, Olindo Assis ; Tafuri, Wagner Luiz ; Reis, Alexandre Barbosa
Date: 2012
Origin: Oasisbr
Subject(s): Trypanosoma cruzi; Chagas' disease; Canine model; Class and subclass of immunoglobulins
A detailed investigation has been carried out about the serological profiles of groups of dogs experimen-tally infected with metacyclic (MT) or blood (BT) trypomastigotes of Berenice-78 Trypanosoma cruzi strain. Peripheral blood was collected from infected dogs and uninfected controls, weekly during 35 days following the acute phase of infection, and immunoglobulin profiles were determined by ELISA. Dogs infected with BT exhibited unaltered levels of IgG2, increases in IgM, IgE, IgA, IgG and IgG1. In contrast, dogs infected with MT presented unaltered levels of IgE and IgG1 and an increase in IgM, IgA, IgG and IgG2 levels. Compared with the MT group, animals infected with BT showed significant increases in IgM on days 7, 14 and 28, in IgA on days 7, 14 and 21, in IgE on days 7 and 14, in IgG on days 14 and 28, and in IgG1 on days 7, 14 and 21. Parasitemia levels of the infected animals were measured over the same time period. No correlations were found between the immunoglobulin profiles and the parasi-temia levels. The results demonstrated that the inoculum source (BT or MT) influence the immunoglob-ulin isotype profile that may drive distinct outcome of acute canine Chagas disease
